The BCL method for elucidating a protein fold using Cryo-EM data has been applied to human adenovirus protein IIIa. This protein, for which there is no high resolution structure, is predicted to be highly α-helical. It is resolved in a 6.8Å resolution cryoEM adenovirus structure as a bundle of 14 α-helical density rods. A partial model where 11 out of 14 α-helices are predicted with a high confidence has been built for this crucial adenovirus capsid protein.
Alumni Project Members: Steffen Lindert