Rosetta Workshop 2024

A workshop for protein design with Rosetta and associated tools was held July 22nd to July 24th, 2024. The tutorials for the workshop consisted of both introductory presentations as well as work-at-your-own-pace exercises.

The workshop materials are written for Rosetta version 3.14

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Introductory Presentations:

• Introduction by Jens Meiler (pdf)
• Rosetta IO & Overview (pdf)
• RosettaScripts (pdf)
• Rosetta Energy Function (pdf)

Main Tutorials:

Tutorial 1: Machine Learning Structure Prediction (RoseTTAFold, AlphaFold, and Colabfold)

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)

Tutorial 2: Machine Learning Protein Design in Rosetta

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)

Tutorial 3: RFDiffusion

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)

Tutorial 4: Immunogen Design (with Torben Schiffner)

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)

Tutorial 5: Enzyme Design with RFdiffusionAA and LigandMPNN

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)

Tutorial 6: Macrocyclic peptide design with Noncanonical Amino Acids

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)