Rosetta Workshop March 2025

A workshop for protein design with Rosetta and associated tools was held March 3rd to March 5th, 2025. The tutorials for the workshop consisted of both introductory presentations as well as work-at-your-own-pace exercises.

The workshop materials are written for Rosetta version 3.14

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Introductory Presentations:

• Introduction by Jens Meiler (pdf)
• Rosetta IO & Overview (pdf)
• RosettaScripts (pdf)
• Rosetta Energy Function (pdf)

Main Tutorials:

Tutorial 1: RFDiffusion

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)

Tutorial 2: Machine Learning Protein Design in Rosetta

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)

Tutorial 3: Protein-Protein Docking

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)

Tutorial 3: Ligand Docking

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)

Tutorial 5: Enzyme Design with RFdiffusionAA and LigandMPNN

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)

Tutorial 6: Macrocyclic peptide design with Noncanonical Amino Acids

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)