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ledwitkv
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Research Assistant Professor - k.ledwitch@vanderbilt.edu
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- Integrative modeling
- Ion channels
- Membrane mimetics
- Membrane proteins
- Small-angle neutron scattering
- Structure determination
- Paramagnetic resonance
- Protein NMR
- Transporters

I develop biophysical technologies to elucidate the structure of membrane proteins (MPs) in lipid environments. Specifically, I am developing an experimental strategy that gives simultaneous access to several long-range distance and orientational restraints (PCSs, RDCs and PREs) by NMR through a noncanonical amino acid paramagnetic labelling strategy. These sparse experimental datasets are integrated with a computational RosettaNMR framework to drive MP structure prediction. This integrated framework is being benchmarked on a model MP system, the four-helical bundle DsbB from E. Coli. To further demonstrate the utility of this approach, I have successfully transitioned this technology from detergent micelles to circularized nanodiscs loaded with different lipid compositions. This work has also piloted complementary investigations using small-angle neutron/X-ray scattering to probe perturbations in the 3D-spatial representation of DsbB under different membrane mimetic environments.