Rosetta Workshop November 2025

A workshop for protein design with Rosetta and associated tools was held November 17th to 19th, 2025. The tutorials for the workshop consisted of both introductory presentations as well as work-at-your-own-pace exercises.

The workshop materials are written for Rosetta version 3.15

*If you experience difficulties downloading the following content, please try again on a different or updated browser.

Introductory Presentations:

• Introduction by Jens Meiler (pdf)
• Rosetta & RosettaScripts Overview (pdf)
• Rosetta Energy Function (pdf)

Main Tutorials:

Tutorial 1: RFDiffusion

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)

Tutorial 2: Machine Learning Protein Design in Rosetta

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)

Tutorial 3: Protein-Protein Docking

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)

Tutorial 3: Ligand Docking

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)

Tutorial 5: Enzyme Design with RFdiffusionAA and LigandMPNN

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)

Tutorial 6: Macrocyclic peptide design with Noncanonical Amino Acids

• Presentation (pdf)
• Tutorial (pdf)
• Materials (zip)